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MyoD targets TAF3/TRF3 to activate myogenin transcription.

Molecular cell | October 13, 2008 |

Deato, Maria Divina E | Marr, Michael T | Sottero, Theo | Inouye, Carla | Hu, Ping | Tjian, Robert
Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.

Abstract

Skeletal muscle differentiation requires a cascade of transcriptional events to control the spatial and temporal expression of muscle-specific genes. U ... of labor between activators and TAF coactivators, thus providing another strategy to accommodate cell-specific regulation during metazoan development.

Skeletal muscle differentiation requires a cascade of transcriptional events to control the spatial and temporal expression of muscle-specific genes. Until recently, muscle-specific transcription was primarily attributed to prototypic enhancer-binding factors, while the role of core promoter recognition complexes in directing myogenesis remained unknown. Here, we report the development of a purified reconstituted system to analyze the properties of a TAF3/TRF3 complex in directing transcription initiation at the Myogenin promoter. Importantly, this new complex is required to replace the canonical TFIID to recapitulate MyoD-dependent activation of Myogenin. In vitro and cell-based assays identify a domain of TAF3 that mediates coactivator functions targeted by MyoD. Our findings also suggest changes to CRSP/Mediator in terminally differentiated myotubes. This switching of the core promoter recognition complex during myogenesis allows a more balanced division of labor between activators and TAF coactivators, thus providing another strategy to accommodate cell-specific regulation during metazoan development.

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Fig 1. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.
Nihms74824f1
Fig 1. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.

Fig 1

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Fig 2. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.
Nihms74824f2
Fig 2. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.

Fig 2

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Fig 3. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.
Nihms74824f3
Fig 3. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.

Fig 3

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Fig 4. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.
Nihms74824f4
Fig 4. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.

Fig 4

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Fig 5. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.
Nihms74824f5
Fig 5. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.

Fig 5

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Fig 6. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.
Nihms74824f6
Fig 6. Deato, Marr, et al. "MyoD targets TAF3/TRF3 to activate myogenin transcription." Molecular cell 32.1 (2008): 96-105. Web.

Fig 6

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