Pdf

Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture.

PloS one | October 03, 2010 |

DeQuach, Jessica A | Mezzano, Valeria | Miglani, Amar | Lange, Stephan | Keller, Gordon M | Sheikh, Farah | Christman, Karen L
DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.

Abstract

The native extracellular matrix (ECM) consists of a highly complex, tissue-specific network of proteins and polysaccharides, which help regulate many c ... create tissue-specific ECM coatings, which offer a promising platform for cell culture to more closely mimic the mature in vivo ECM microenvironment.

The native extracellular matrix (ECM) consists of a highly complex, tissue-specific network of proteins and polysaccharides, which help regulate many cellular functions. Despite the complex nature of the ECM, in vitro cell-based studies traditionally assess cell behavior on single ECM component substrates, which do not adequately mimic the in vivo extracellular milieu.We present a simple approach for developing naturally derived ECM coatings for cell culture that provide important tissue-specific cues unlike traditional cell culture coatings, thereby enabling the maturation of committed C2C12 skeletal myoblast progenitors and human embryonic stem cells differentiated into cardiomyocytes. Here we show that natural muscle-specific coatings can (i) be derived from decellularized, solubilized adult porcine muscle, (ii) contain a complex mixture of ECM components including polysaccharides, (iii) adsorb onto tissue culture plastic and (iv) promote cell maturation of committed muscle progenitor and stem cells.This versatile method can create tissue-specific ECM coatings, which offer a promising platform for cell culture to more closely mimic the mature in vivo ECM microenvironment.

High Discussion
Low Discussion
Paper
Fig 1
Fig 2
Fig 3
Fig 4
Fig 5
Fig 6

Summary of The Overall Paper

Click to enter summary

Sign up to leave a summary.


Sign up to leave a summary.



Comments
Pone.0013039.g001
Fig 1. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.
Pone.0013039.g001
Fig 1. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.

Fig 1

Click to enter summary

Sign up to leave a summary.


Click to list methods

Sign up to leave a summary.



Comments
Pone.0013039.g002
Fig 2. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.
Pone.0013039.g002
Fig 2. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.

Fig 2

Click to enter summary

Sign up to leave a summary.


Click to list methods

Sign up to leave a summary.



Comments
Pone.0013039.g003
Fig 3. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.
Pone.0013039.g003
Fig 3. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.

Fig 3

Click to enter summary

Sign up to leave a summary.


Click to list methods

Sign up to leave a summary.



Comments
Pone.0013039.g004
Fig 4. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.
Pone.0013039.g004
Fig 4. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.

Fig 4

Click to enter summary

Sign up to leave a summary.


Click to list methods

Sign up to leave a summary.



Comments
Pone.0013039.g005
Fig 5. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.
Pone.0013039.g005
Fig 5. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.

Fig 5

Click to enter summary

Sign up to leave a summary.


Click to list methods

Sign up to leave a summary.



Comments
Pone.0013039.g006
Fig 6. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.
Pone.0013039.g006
Fig 6. DeQuach, Mezzano, et al. "Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture." PloS one 5.9 (2010): e13039. Web.

Fig 6

Click to enter summary

Sign up to leave a summary.


Click to list methods

Sign up to leave a summary.



Comments