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A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library.

ACS nano | October 25, 2010 |

Wang, Hao | Liu, Kan | Chen, Kuan-Ju | Lu, Yujie | Wang, Shutao | Lin, Wei-Yu | Guo, Feng | Kamei, Ken-ichiro | Chen, Yi-Chun | Ohashi, Minori | Wang, Mingwei | Garcia, Mitch André | Zhao, Xing-Zhong | Shen, Clifton K-F | Tseng, Hsian-Rong
Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.

Abstract

Nanoparticles are regarded as promising transfection reagents for effective and safe delivery of nucleic acids into a specific type of cells or tissues ... DNA⊂SNPs. We envision such a rapid developmental pathway can be adopted for generating nanoparticle-based vectors for delivery of a variety of loads.

Nanoparticles are regarded as promising transfection reagents for effective and safe delivery of nucleic acids into a specific type of cells or tissues providing an alternative manipulation/therapy strategy to viral gene delivery. However, the current process of searching novel delivery materials is limited due to conventional low-throughput and time-consuming multistep synthetic approaches. Additionally, conventional approaches are frequently accompanied with unpredictability and continual optimization refinements, impeding flexible generation of material diversity creating a major obstacle to achieving high transfection performance. Here we have demonstrated a rapid developmental pathway toward highly efficient gene delivery systems by leveraging the powers of a supramolecular synthetic approach and a custom-designed digital microreactor. Using the digital microreactor, broad structural/functional diversity can be programmed into a library of DNA-encapsulated supramolecular nanoparticles (DNA⊂SNPs) by systematically altering the mixing ratios of molecular building blocks and a DNA plasmid. In vitro transfection studies with DNA⊂SNPs library identified the DNA⊂SNPs with the highest gene transfection efficiency, which can be attributed to cooperative effects of structures and surface chemistry of DNA⊂SNPs. We envision such a rapid developmental pathway can be adopted for generating nanoparticle-based vectors for delivery of a variety of loads.

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Nihms243367f1a
Fig 1. Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.
Nihms243367f1a
Fig 1. Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.

Fig 1

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Fig 2. Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.
Nihms243367f2
Fig 2. Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.

Fig 2

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Fig 3. Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.
Nihms243367f3
Fig 3. Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.

Fig 3

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Fig 4. Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.
Nihms243367f4
Fig 4. Wang, Liu, et al. "A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library." ACS nano 4.10 (2010): 6235-43. Web.

Fig 4

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